{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lectures 12 & 13 amended -Tissue culture and plant propagation

Lectures 12 & 13 amended -Tissue culture and plant propagation

Info icon This preview shows pages 1–26. Sign up to view the full content.

View Full Document Right Arrow Icon
Tissue Culture and Plant Propagation Hartmann et al. – Chapters 17 and 18 Mike Hasegawa (HORT 318) – [email protected] . Plant tissue culture - plant, organs, tissues or cells grown in vitro Nutrient medium – essential mineral nutrients, carbon source and plant growth regulators Controlled environmental conditions - light, temperature, humidity Why is tissue culture relevant to plant propagation? Disease eradication, rapid clonal multiplication of plants, plants from immature embryos, biotechnology 1
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
1. Plantlet formation- Roots and shoots arise from explant 1. Callus and cells- Callus and cells 1. Seedling culture- Seedlings develop from embryos in vitro 1. Embryo culture- Embryos made from vegetative tissues Types of structures formed: 2
Image of page 2
1. Plantlet production: Direct- Axillary shoot formation shoot meristem culture shoot tip culture node culture Indirect - Adventitious shoot formation leaf, root, flower, petiole, callus 2. Seedling culture 3. Callus and cells 4. Embryo culture 3
Image of page 3

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
            Meristem & Shoot Culture                   Node Culture  4
Image of page 4
1. Plantlet Formation - Axillary shoots develop from meristem or shoot tips Leaf Primordia 5
Image of page 5

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
1. Plantlet production: Axillary shoot formation = “micropropagation” meristem culture – disease free shoot tip culture node culture Adventitious shoot formation   leaf, root, flower, petiole, callus 2. Seedling culture 3. Callus culture 4. Embryo culture 6
Image of page 6
Meristem culture to obtain disease-free stock plants 7
Image of page 7

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Heat therapy to eradicate virus 1. Shoots  in vitro   2. growth chamber 3. 3-6 weeks 4. 100 o F 5. meristems harvested Plantlet formation  – tissue culture for disease-free stock 8
Image of page 8
1. Plantlet production: Axillary shoot formation = “micropropagation” shoot meristem culture shoot tip culture node culture Adventitious shoot formation   leaf, root, flower, petiole, callus 2. Seedling culture 3. Callus culture 4. Embryo culture 9
Image of page 9

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Shoot propagation of asparagus by enhanced axillary shoot development Six (6) shoots each passage Six (6) passages/year = 46656 plants/yr 10 Cytokinin promotes axillary shoot development
Image of page 10
Shoot apical dominance – auxin induced and cytokinin antagonized Cytokinin promotes lateral bud development 11
Image of page 11

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Asparagus shoot multiplication after one passage 12
Image of page 12
Shoot multiplication and root formation of asparagus 13
Image of page 13

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Node culture 14 Cytokinin stimulates lateral bud development and multiplication
Image of page 14
Lily shoot multiplication 15
Image of page 15

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Tissue culture multiplication 16
Image of page 16
Hardening of plants for soil transplantation 17
Image of page 17

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
18
Image of page 18
Mist bench for acclimation to soil 19
Image of page 19

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
20
Image of page 20
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern