Midterm II Fall 10 - Answer Key

Midterm II Fall 10 - Answer Key - Stimulus AA Stimulus BB...

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Stimulus A B C D Gene Z B Stimulus A B Stimulus A B C D Gene Z LacZ MCS Amp R Ori P BIS101-01, Fall 2010 Dr. D. J. Kliebenstein, Instructor Midterm Exam II Please print your name and ID number on page 2 and the rest of the exam. Do not print your ID number on the first page. The exam has 10 questions and a total of 120 points For full credit, show all of your work. AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I,_________________________________, Authorize the University to publicly distribute my graded exam (e.g., handed out in class or left in an exam bin) for BIS101-01. If I do not sign, then I must see the instructor to pick up my exam. Signed:_______________________________________ Dated:__________________________________
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1. __________ 2. __________ 3. __________ 4. __________ 5. __________ 6. __________ 7. __________ 8. __________ 9. __________ 10. __________ Total _________
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1. (18 points) Complete the following Table, assuming that the DNA is written with the top left being a 5’ end and that the columns represent transcriptional and translational alignments. For DNA, use ACTG or N where N represents any possible nucleotide. The RNA is drawn in the direction that it is read from the DNA. T G G A C A C A T G A T DNA Double Helix A C C T G T G T A C T A A C C U G U G U A C U A mRNA U G G A C A C A U G A U tRNA Anticodon PRO CYS MET ILE Amino Acids Which of the above rows is the coding strand? The second DNA strand is the coding strand. The bottom table is typically thought of as describing the tRNA’s, are there any codons that do not correspond to tRNA’s? If so, what are they and what do they do? The UAA, UAG and UGA codons do not represent tRNA’s and they cause translation to stop.
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2. We discussed two types of donor DNA that can be used to make libraries to find the DNA for a gene. (8 pts) a. What are these two types of DNA and where did they come from? These are genomic DNA that was isolated from the genome and cDNA which was made from RNA b. Which type of library would you use for functional complementation when studying a eukaryotes genome? cDNA library. c. How would you change the protocol for making the correct library if you were interested in studying ribosomal RNA from a eukaryote? You would use a cDNA library except that you wouldn’t use polyA RNA as that is only for mRNA not rRNA. 3. Eukaryotes have the problem of having to pack a ton of DNA into an itsy bitsy teeny tiny space. We discussed how this occurred via multiple sequential levels of DNA packaging. What are the four levels of DNA packaging that we talked about in the order from least packaged to most packaged. Also point out the two forms that are important in regulation and give their other name that we discussed in regulation (8 pts)
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This note was uploaded on 04/04/2011 for the course BIS 101 taught by Professor Simonchan during the Fall '08 term at UC Davis.

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Midterm II Fall 10 - Answer Key - Stimulus AA Stimulus BB...

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