chromatographer

chromatographer - 1 Chem 341 - Chromatography, Copyright -...

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Unformatted text preview: 1 Chem 341 - Chromatography, Copyright - D.J. Dyer & SIUC Chromatography: Thin-Layer Chromatography (TLC) & Column Chromatography Important Concepts • General Theory of Chromatography • DeFnitions: R ¡ , eluant, eluate, stationary phase, adsorbent, mobile phase • TLC technique • Column Chromatography Part 1, p. 180: Separation of spinach pigments by TLC. Part 2, p. 188: Separation of ¡luorene and ¡luorenone by column chromatography. 2 Chem 341 - Chromatography, Copyright - D.J. Dyer & SIUC Theory of Chromatography Chromatography - a technique to separate and purify organic compounds. Theory - The components of a mixture will interact differently with the solid support such that one component moves faster than the other. Mobile Phase - consists of a liquid or gas that Fows continuously. Stationary Phase - a solid support that typically remains heterogeneous; for gas chromatography the stationary phase is a solid support coated with a viscous liquid resin.- Organic compounds will exhibit different af¡nity for the mobile phase vs. stationary phase. Thus, an equilibrium is established between the two phases with a partition coef¡cient ( K ). Since K will be different for each component (or eluate ) of a mixture, they will separate out into migratory bands , which Fow through the system. 3 Chem 341 - Chromatography, Copyright - D.J. Dyer & SIUC Theory of Chromatography - cont. Adsorbent - the solid phase (stationary phase), which is typically very polar. Silica gel (SiO 2 ) x or Alumina (Al 2 O 3 ) x are the most common.- polar molecules stick to the adsorbent more tightly than nonpolar molecules. Eluate - the compound that we are trying to separate and purify. Eluant - the mobile phase, usually an organic solvent or mixture of solvents. As the solvent Fows through the adsorbent, the molecules will become detached from the stationary phase and move with the mobile phase....
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This note was uploaded on 04/04/2011 for the course CHEM 341 taught by Professor Staff during the Fall '08 term at SIU Carbondale.

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chromatographer - 1 Chem 341 - Chromatography, Copyright -...

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