anat 262 cyto skel slides

anat 262 cyto skel slides - ANAT 262 Cytoskeleton Lectures...

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Unformatted text preview: ANAT 262 Cytoskeleton Lectures Plan of Attack Day 1-- Overview of Cytoskeleton Microtubules, Actin, Intermediate Filaments. Day 2 -- Microtubules Polymerization, cilia, centrioles, dynein, kinesin Day 3 -- Actin Organization, Arp 2/3, rho-gtpases, myosins Day 4 -- Intermediate Filaments and Cell Motililty Dr. Craig Mandato (craig.mandato@mcgill.ca) Cytoskeleton 3 Components: 1. Microtubules 2. Actin 3. Intermediate Filaments Main Functions: 1) Structure and Support Determination of Cell Shape Establishing Intercellular Contacts 2) Intracellular Transport 3) Contraction and Motility Cell Division 4) Spatial Organization Signal Transduction Tensile Strength The term CSK may in one sense have an unfortunate connotation since it implies that the various structural elements making up the cytoskeleton form a static structural framework similar to the bone skeleton that frames the body. Well this only partly true because certain types of cytoskeleton elements are more or less permanent features of the cell. Examples such CSK structures include the actin and myosin Flament bundles in muscle cells and the microtubule arrays in cilia and agellae. Other CSK structures are very dynamic, continuously assembling and disassembling as part of their functional cycle or for use in various cellular processes. Perhaps the most dramatic example of such a dynamic process is the complete remodeling of the microtubule array of a cell during mitosis when it changes from a network radiating throughout the cell to a compact, bipolar, mitotic spindle. Handout #1 F-Actin ~ 8nm in diameter and is highly concentrated at the cell cortex and is associated with the plasma membrane. Looks like 2 chains twisted together, but this is just the way actin polymerizes Microtubules ~ 25nm in diameter and is a hollow tube made of tubulin. There are 13 laterally associated protolaments, each being a linear polymer. One end of the MT is attached to the centrosome Intermediate Filaments ~ 10nm in diameter, a complex assembly of tetramers made from subunits. Various types of IF subunits over 50 cytoplasmic IFs and Nuclear Lamins. Ex. Desmosomes in epithelial cells. The basic structural elements of the CSK AFs, MTs and IFs do not act alone There is a multitude of CSK-associated proteins that anchor, cross-link and otherwise regulate these major elements. The ability of actin, tubulin and IF proteins to form polymers, to depolymerize, to interact, elongate and contract in conjunction with subtle cell movements and shape changes gives the cytoplasm its unique properties of life The organized arrays of brous elements interact to form a highly integrated structural network we refer to as the CSK. This CSK gives the cytoplasm its unique properties AFs, MTs are found in all eukaryotic cells Tubulin is generally present in smaller amounts then actin, except in neurons, where tubulin is very abundant....
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This note was uploaded on 04/04/2011 for the course ANAT 261 taught by Professor Morales&mandato during the Spring '08 term at McGill.

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anat 262 cyto skel slides - ANAT 262 Cytoskeleton Lectures...

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