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Old test 1 key - Mic102/Fa09/Appleman Quiz 1 21 October...

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Mic102/Fa09/Appleman Quiz 1, 21 October 2009 This quiz is to represent only your own work . As a UC Davis student, you are bound by an honor code; if you can’t be trusted with that, can you be trusted in a career in, say, the health professions? Not only does this honor code demand personal integrity, but it also requires you to demand integrity from your peers. This quiz is closed book, closed note. Please hand in a blue scantron with the answers to the multiple-choice section of the quiz. Make sure that it has your name, student I.D.#, and Test form A ” filled in. You will also need to hand in the last two pages of this quiz, with your name on each. Much has been learned in the last few years about syntrophic collaborations between microbes, and how they allow complete exploitation of energy-poor environments. Analysis of the metabolism of these organisms has taught us interesting things about energy generation, and analysis of the composition of these cells has taught us amazing things about the fine structure of Bacterial and Archaeal cells. A model system for the study of syntrophy has been the collaboration between the fermenting Bacterium Clostridium kluyveri and the methanogenic Archaeon M. bryantii . The genome of Clostridium kluyveri has been sequenced, and is full of interesting stuff… “C lostridium kluyveri was first enriched by H. A. Barker in 1937 from the mud of a canal in Delft, The Netherlands. The enrichment culture fermented ethanol to acetate, butyrate, caproate, and methane. From the enrichment two organisms growing on ethanol were isolated. One organism, named C. kluyveri , ferments ethanol and acetate to butyrate, caproate, and H 2 ; toward the end of growth also butanol and hexanol are formed. The other organism, named Methanobacillus omelianskii , fermented ethanol and CO 2 to acetate and methane. M. omelianskii was later found to be a coculture of a bacterium fermenting ethanol to acetate and H 2 (S-organism) and an archaeon reducing CO 2 with H 2 to methane [ Methanobacterium bryantii ]. All three organisms came to fame, C. kluyveri as a model organism in the early 1950s for the study of fatty acid synthesis and fatty acid oxidation, and the S-organism and M. bryantii for the development of the concept of interspecies hydrogen transfer and of syntrophy.” --Proceedings of the National Academy of Sciences, 12 February 2008 ( 105: 6, 2128)
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1. C. kluyveri is a member of the Gram-positive group of the Bacteria. Which of these techniques is NOT useful for placing it in this group? a) transmission EM—can identify thick cell wall b) phase-contrast microscopy c) Gram staining—obviously good for Gram + d) analysis of rRNA sequence—the ultimate way to classify life
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