Review Questions for lecture 13

Review Questions for lecture 13 - Review Questions for...

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Review Questions for lecture 13 Be comfortable with the following terms. Bear in mind that you may have to look some of them up in a textbook. It is expected that you have access to some sort of textbook for this class. There is no specific textbook that is required, but you will need to have some sort of resource in addition to lectures. Feedback regulation Negative Feedback Positive Feedback Heat-Shock Response Alternative Sigma factor Protease (DnaK is an example of one) “Selfish DNA” Plasmid High copy-number plasmid Addiction Module (Toxin-Antitoxin module) Transposon Intron Conjugative Plasmid F Element, F’, Male/female F Pilus Tra genes Rolling Circle Replication Virus Phage Capsid/Coat protein Receptor Filamentous virus (e.g. M13 or fd) Coat protein RNA virus (Q β or MS2—they are very similar) RNA-templated RNA polymerase ssDNA virus (e.g. φ X174) Alternative reading frame “Leaky” terminator Lytic phage dsDNA phage (e.g. T4 or “T-even”) Timed gene expression/”regulatory cascade Questions from class: Arrange the following in order of their relative abundance in the cell: chromosome, gene, operon, transcription terminator, promoter, regulon. Design a genetic element that turns itself off. Now design another one. Now design another one. There are many ways to do this! Given two otherwise identical cells, which will grow more rapidly: one with 5000 non- coding bases of DNA, or one without the extra DNA? Why? What happens to cells with “extra” DNA in them over evolutionary time? Which is more accurate: “Cells have a mechanism for ridding themselves of extra DNA”, or “Life have a way of ridding itself of cells that have extra DNA”? What are some examples of feedback in your daily life? Why do college students comply with written instructions saying “please” and “thank you,” even though those instructions may not be beneficial to said students? Design a genetic system that, when introduced into a cell, will express one gene, then
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This note was uploaded on 04/05/2011 for the course MIC 102 taught by Professor Appleman during the Fall '08 term at UC Davis.

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Review Questions for lecture 13 - Review Questions for...

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