Review Questions for Lecture 18

Review Questions for Lecture 18 - Review Questions for...

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Review Questions for Lecture 17 Be comfortable with the following terms: Enrichment culturing Metagenomics FISH Isotopic labeling Symbiosis Mutualism Parasitism Commensalism Syntrophy Legume Nodule NodD Nod Factor Megaplasmid Leghemoglobin Nod factor Endosymbiosis Bacteroid If you were presented with a mixture of organisms from a rich environment, how would you isolate autotrophs? Azotrophs? Phototrophs? Photoautotrophs? In class, we discussed an enrichment experiment that looked for nitrite-using photolithoautotrophs. What are a couple of ways you could look for autotrophs? For Photoautotrophs? For Nitrite users? What are a couple of ways you could get phylogenetic information about these organisms? Often, enrichment cultures produce mixtures of organisms—two or three types of cells. In such a situation, how can you tell which cells are doing the job you’re interested in (e.g. fixing nitrogen)? Which would be more useful: FISH or isotopic labeling, or are they both fine? If you know the metagenome of an environment, do you know for sure what is being done in that environment? Do you have any clue as to who is doing a particular job in that environment? Differentiate between symbiosis as used colloquially and scientifically. Does a symbiosis always benefit both partners? The genes for all Nod functions in Rhizobium are found on a megaplasmid, about 2 Mb (million bases) in size. Is symbiosis essential for Rhizobium ? Is it essential for the legume?
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This note was uploaded on 04/05/2011 for the course MIC 102 taught by Professor Appleman during the Fall '08 term at UC Davis.

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Review Questions for Lecture 18 - Review Questions for...

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