Unformatted text preview: C mL/min 12 = Cr P mg/L (for men) or 10 mg/L (for women) 2. calculate dilution factor (X) needed for Cr U ≅ 30 mg/L from: L mg L mg Cr U X 30 = 3. Round X to the nearest (convenient) whole number (when in doubt, round up) 4. prepare blank and dilution samples (see lab manual for details) 5. Measure absorbance (OD) of the blank and all diluted samples 6. Convert absorbance to urine-creatinine concentration using calibration curve. The units of the calibration curve are mg/dL ⇒ convert to mg/L by multiplying by 10 dL/1 L 7. Multiply answer from step 6 by dilution factor from step 3 to obtain undiluted Cr U value 8. Calculate the true creatinine clearance as: Cr Cr Cr P V U C o ⋅ = Where, Cr P is from step 1, Cr U is from step 7, and = o V (urine volume)/(time elapsed)...
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- Spring '08
- Calculation, calibration curve, 0 min, Urine flow rate