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BIS104_F10_Lecture3

BIS104_F10_Lecture3 - Welcome to BIS 104(1 F2010 Instructor...

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Welcome to BIS 104(1), F2010 ± Instructor: ¾ Dr. Bo Liu 2165 Life Sciences e-mail: [email protected] Office Hours: WF 3 - 4 pm ± Teaching Assistant: ¾ Cui (Tracy) Zeng 2061 Science Laboratory Building e-mail: [email protected] Office Hours: MT 4 - 5 pm Announcements: Friday’s office hour: 11 – noon Textbooks in reserve
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Fluorescent microscopy in breast cancer prognosis Roche Herceptin (trastuzumab): a monoclonal antibody
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v. Polyclonal antibodies vs. monoclonal antibodies Each antigen may have many antigenic determinants: epitopes Each B cell only secrets identical Igs Polyclonal antibodies: mixture of antibodies (Igs)- recognizing different epitopes on an antigen Monoclonal antibodies: derived from single B cells and its progeny-recognizing a single epitope
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How did Kohler and Milstein achieve the goal of producing monoclonal antibodies, and win Nobel prize? Prof. Peter Hepler, UMass-Amherst Preparation of hybridomas that secrete monoclonal antibodies against a particular antigen
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Prof. Peter Hepler, UMass-Amherst b. Separating proteins i. Fractionation of cell contents by differential centrifugation
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ii. Gradient centrifugation
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charges, and affinity to others
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BIS104_F10_Lecture3 - Welcome to BIS 104(1 F2010 Instructor...

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