BIS104_F10_Lecture14m

BIS104_F10_Lecture14m - BIS104 (1), Fall 2010 Topic VII:...

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BIS104 (1), Fall 2010 Topic VII: Cell Division Cycle 1. Dissecting cell division cycle 2. Maturation promoting factor (MPF) and Cyclin- dependent kinase 3. Cell cycle checkpoints 4. Cytoskeleton and cell division 5. Cytokinesis
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¾ Cell cycle is divided into different phases
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1. Dissecting cell division cycle i. Cytological (microscopic) observations ¾ Cells at M phase are easily observed ¾ Cultured cells have doubling times of ~20 hrs ¾ ~5% of cultured cells are at M phase ¾ ~1 hr to complete the M phase
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ii. Labeling S phase cells with thymidine 3 H labeled thymidine The thymidine analog BrdU The length of S phase = % of S phase cells (labeled) X length of one cell cycle
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iii. Measuring DNA contents inside cells with a flow cytometer DNA is labeled with specific (fluorescent) dyes like DAPI Measuring fluorescence signal in individual cells Can even be used to sort cells: FACS (fluorescence-activated cell sorter)
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iv. Genetic dissection of cell division cycle in fungi The mold Aspergillus nidulans (C)
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¾ Temperature sensitive (ts) mutations 9 L. Hartwell isolated cdc mutants in the budding yeast
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This note was uploaded on 04/05/2011 for the course BIS 104 taught by Professor Scholey during the Fall '08 term at UC Davis.

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BIS104_F10_Lecture14m - BIS104 (1), Fall 2010 Topic VII:...

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