BIS104_F10_SampleQs

BIS104_F10_SampleQs - BIS104_F10_Liu_Sample Test 1...

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BIS104_F10_Liu_Sample Test 1 10/08/2010 1 Sample test 1. The stereocilia projecting from the inner ear of an animal are loaded with mechanically gated ion channels. It is hypothesized that a hearing defect caused by a genetic disease was due to the failure of the channel protein to be associated with the plasma membrane. In order to test whether the protein is plasma membrane-associated or diffuses in the cytosol, which of the following microscopic approaches will most likely be applied? A. Nomarski differential interference contrast (DIC) microscopy using live samples B. Phase contrast microscopy using live samples C. Fluorescence microscopy using live samples D. Scanning electron microscopy (SEM) using live samples E. Transmission electron microscopy (TEM) using live samples 2. The jellyfish green fluorescent protein (GFP) can be excited by blue light at ~450 nm and emits green fluorescence at ~510 nm. The dsRed protein isolated from reef coral emits red fluorescence at ~583 nm when excited by green light at ~550 nm. By recombinant DNA and transgenic means, a protein of interest can be expressed in fusion with GFP or dsRed so that the protein can be monitored in live cells. When protein A is fused with GFP and protein B is fused with dsRed, two fluorescent signals can be detected individually using two different combinations of filters and beam splitters (dichroic mirrors). Which of the following beam splitters will be the best choice for observing the A-GFP fusion protein? A. A beam splitter that reflects light at ~380 nm and passes lights between 435 nm and 490 nm B. A beam splitter that reflects light at ~420 nm and passes lights between 465 nm and 500 nm C. A beam splitter that reflects light at ~450 nm and passes lights between 505 nm and 550 nm D. A beam splitter that reflects light at ~510 nm and passes lights between 525 nm and 570 nm E. A beam splitter that reflects light at ~550 nm and passes lights between 575 nm and 620 nm 3. The resolving power (resolution, d) of a light microscope can be calculated in the formula d=0.61 /n sin θ reflecting the contributions of the wavelength of the light ( ) used, the refractive index (n) of the medium separating the specimen from the objective, and the width of the cone of light that
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BIS104_F10_SampleQs - BIS104_F10_Liu_Sample Test 1...

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