Chem 481 F10 HW 10-13-10

Chem 481 F10 HW 10-13-10 - Name_ EnzymeMechanismsAssignemnt

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Name_____________________ Enzyme Mechanisms Assignemnt Chem 481  Dr. Steven Wood You may use your Lehninger text, notes and consult with your peers, but your answers need to be your own i.e. they are not to be formulated by the group and then copied by all members of the group; you need to write out your individual answers. Due: Friday, October 22 nd at 5 pm in the drop box. 1. You are studying genetic disorder or disease that is caused by structural variants of a single enzyme found in the liver. You have isolated and purified this enzyme using your newly acquired knowledge of protein isolation techniques from an individual not afflicted with the malady and from two different individuals who are symptomatic for the disease. In order to characterize the relative catalytic abilities of the three enzymes, you decide to analyze their kinetic profiles. Write out the Michaelis-Menton equation and explain the meaning of each term. 2. Upon the completion of your study above, you analyze of the data and determine the following constants: Enzyme K m k cat Normal Individual 5 X 10 -4 1 X 10 3 Patient 1 5 X 10 -2 1 X 10 3 Patient 2 5 X 10 -4 1 X 10 1 Show how k cat is related to a term(s) in the Michealis-Menton equation. What does the higher K
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This note was uploaded on 04/06/2011 for the course CHEM 481 taught by Professor Wood during the Fall '10 term at BYU.

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Chem 481 F10 HW 10-13-10 - Name_ EnzymeMechanismsAssignemnt

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