BIS 104 PQ 7 ans spring 2010

BIS 104 PQ 7 ans spring 2010 - ~30,000 Da Express your...

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BIS 104 PQ 7 ans spring 2010 COP Ia is a protein that is found in the cytoplasm of most eukaryotic cells. Investigators desiring to know more about the function of this protein decided to isolate and purify the protein from HeLa cells. HeLa cells are an immortal line of human cancer cells that are easily grown in large quantities in cell culture. In describing their procedures, these investigators claimed that they were able to obtain 30 micrograms (30 ug) of pure COP Ia from 1 x 10 9 HeLa cells and that, on average, each HeLa cell contained approximately 5 x 10 5 molecules of COP Ia protein. Given the above data, show your work and calculate the following: A. Estimate the molecular weight of the COP Ia protein. (cell / 5 x 10 5 molecules) X (1 / 1 x 10 9 cells) X (3 x 10 -5 g / 1 x 6.02 x 10 23 molecules / mole) = ~ 30,000 g/mole Express your answer in Daltons:
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Unformatted text preview: ~30,000 Da Express your answer in kilodaltons: ~30 kDa B. Estimate the number of amino acids in this protein. (30,000 Da / 1) X (1 amino acid / 120 g) = 250 amino acids C. Given this number of amino acids (answer to B), how many nucleotide bases are in the translatable portion of the mRNA transcript for this protein? 250 amino acids X 3 NTB / aa = 750 NTBs D. How many nucleotide bases are in the sense stand of the gene that codes for COP Ia mRNA? a. Fewer than the number of bases in the mRNA. b. The same number of bases as in the mRNA. c. More than the number of bases in the mRNA. Explain the basis for your answer. Sense strand of genic DNA contains non-coding sequences (e.g., regulatory sequences and introns) that are either not transcribed or are spliced out of the final translatable mRNA....
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