BIS 104 PQ 10 ans Spring 2010

BIS 104 PQ 10 ans Spring 2010 - In contrast, staining for...

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BIS 104 PQ 10 ans Spring 2010 10. In their second series of experiments, Mukouyama, et al . immunostained mouse embryo limb skin samples from 3 different developmental staGes (E13.5, E14.5, and E15.5). They immunostained these tissues for arterial markers (ephrinB2, NP1, CX40), the EC marker PECAM-1 and the PSN marker 2H3. Describe the RESULTS of this experiment. RESULTS: Undifferentiated ECs (PECAM-1) and PSNs (2H3) showed strong fluorescent staining at all three developmental stages (13.5, 14.5, and 15.5 days).
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Unformatted text preview: In contrast, staining for arterial ECs (ephrinB2 and CX40) was non-existent at 13.5 days, detectable at 14.5 days, and strong by day 15.5. State the CONCLUSION that can be drawn from these results. CONCLUSION: Since differentiation and branching of PSNs preceded arterial differentiation by a full day, it is plausible that PSNs are responsible for directing the subsequent differentiation of arterial ECs....
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This note was uploaded on 04/06/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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