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Unformatted text preview: optics). d) refractive index: Refers to the degree to which a substance (air, glass, liquid) causes diffraction of light waves. Denoted as “n”, it is a unit-less value. E.g., refractive index of air = 1.0. e) numerical aperture (NA): What is the relationship between the NA of an objective lens and the useful magnification that can be obtained by that lens? Useful magnification is generally limited to approx 1,000 times that of the NA of that lens. A property of the objective lens. Defined as the product of the refractive index of the medium between the specimen and the objective lens and sin alpha (the light gathering ability of the lens). f) empty magnification: Refers to magnification beyond the resolving power of a microscope. It results in an increase in size of the image, but no increase or improvement in resolution or clarity of detail....
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This note was uploaded on 04/06/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08
- cell biology