Unformatted text preview: Both scanning confocal and deconvolution fluorescence microscopy provide a sharper, more focussed image at different depths through the specimen by subtracting (mechanically in the case of confocal scope and mathmatically in the case of the deconvoluton scope) unfocussed fluorescence at each plane. This process greatly improves the resolution of the image. These sharply focussed images from multiple planes can be stored in a computer and then later “stacked” and assembled into a series of crisp images that can be used to re-contrstuct a 3-D image....
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- Spring '08
- cell biology, focal plane, conventional fluorescence microscopy