BIS 104 PQ 12 ans Spring 2010

BIS 104 PQ 12 ans - Both scanning confocal and deconvolution fluorescence microscopy provide a sharper more focussed image at different depths

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BIS 104 PQ 12 ans Spring 2010 12. Advantages provided by both scanning confocal and deconvolution fluoresence microscopy over conventional fluorescence microscopy. When a whole cell or a section of a tissue is examined under a standard fluorescence microscope, the observer views the specimen at different depths by changing the position of the objective lens to obtain different planes of focus throughout the depth of the specimen. This can result in a rather blurred image since at each focal plane, out of focus fluorescence from above and below the plane is also observed.
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Unformatted text preview: Both scanning confocal and deconvolution fluorescence microscopy provide a sharper, more focussed image at different depths through the specimen by subtracting (mechanically in the case of confocal scope and mathmatically in the case of the deconvoluton scope) unfocussed fluorescence at each plane. This process greatly improves the resolution of the image. These sharply focussed images from multiple planes can be stored in a computer and then later “stacked” and assembled into a series of crisp images that can be used to re-contrstuct a 3-D image....
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This note was uploaded on 04/06/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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