BIS 104 PQ 18 Spring 2010

BIS 104 PQ 18 Spring 2010 - the plasma membrane of arterial...

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BIS 104 PQ 18 spr 2010 18. Using the technique of IHC and fluorescence microscopy, Mukouyama has shown that arterial ECs begin to express ephrinB2 on their plasma membranes sometime between E13 and E15 in mouse limb skin. a. Use SDS-PAGE and western blotting to test the hypothesis that ephrinB2 is present in
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Unformatted text preview: the plasma membrane of arterial ECs on day 15, but not on day 13. b. Describe how you would test the hypothesis that ephrinB2 is an integral membrane protein with both external and internal domains. c. Given the structure and location of ephrinB2, speculate as to what its function might be....
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