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Unformatted text preview: are linked to the heavy chains by intermolecular disulfide binds. To evaluate the purity of your IgG preparation, you perform the following tests: A sample of your IgG prep is run on SDS-PAGE under reducing conditions. A sample of your IgG prep is run on SDS-PAGE under non-reducing conditions. Both gels are stained with Coomassie Blue (CB) and the Rm of each stained band is estimated by comparison with a standard curve of proteins with known mol wts. a. Diagram the gel lanes and CB banding patterns you would expect to see from a pure IgG prep. b. Describe how you could use SDS-PAGE and western blotting to verify the specificity of your anti-PECAM antibody. c. Describe how you would use a sample of the rabbit pre-immune serum as a control....
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This note was uploaded on 04/06/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08
- cell biology