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BIS 104 PQ 20 Spring 2010

BIS 104 PQ 20 Spring 2010 - anchored protein Describe an...

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BIS 104 PQ 20 Spring 2010 20. Let’s suppose that you have recently discovered an extraordinarily simple cell that has only two different proteins associated with its plasma membrane. Preliminary analysis shows that protein “A” has a mol wt of 45,000 Da and protein “B” has a mol wt of 60,000 Da. You hypothesize that protein “A” is an external peripheral protein and that protein “B” is a GPI-
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Unformatted text preview: anchored protein. Describe an experimental protocol that would test this hypothesis. Describe the results you would obtain if your hypothesis were shown to be correct. Do you think that freeze-fracture followed by SEM would add any useful information about these proteins? Why, or why not?...
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