BIS 104 PQ 21 ans spr 2010

BIS 104 PQ 21 ans spr 2010 - specific marker SCG-10, but...

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BIS 104 PQ 21 ans spr 2010 21. After separating Schwann cells from neurons by the FACS technique, Mukouyama attempted to verify the purity of these two populations by RT-PCR and gel electrophoretic analysis of the PCR products. Are you convinced that the FACS procedure successfully separated Schwann cells and neurons into two highly purified populations? Explain the basis for your answer. Yes, the data are convincing. Using RT-PCR (Fig. 6D) they show that the purified neuron population expresses the neuron-
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Unformatted text preview: specific marker SCG-10, but not GFAP. In contrast, the purified Schwann cells express only their specific marker, GFAP, and show no expression of SCG-10. Therefore, at the molecular level of analysis for specific mRNA, the populations appear to be greater than 95% pure (i.e., less than 5% cross contamination between the two cell types....
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