BIS 104 PQ 22 ans Spr 2010

BIS 104 PQ 22 ans Spr 2010 - expansion of pre-existing...

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BIS 104 PQ 22 ans Spr 2010 22. When PECAM-1 positive / ephrinB2-negatvie undifferentiated ECs were treated with 2.5 pM pure VEGF protein, approximately 50% of the ECs responded by differentiating (they began expressing ephrinB2). This treatment with VEGF did not lead to any net change in the total number of ECs. What may be concluded from these results? The observed effect of VEGF on ECs is a true induction of their differentiation. The increased percentage (from <5% to 50%) of ephrinB2-positive cells is not due to selection and clonal
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Unformatted text preview: expansion of pre-existing ephrinB2-positive cells. When undifferentiated ECs were treated with higher concentrations of VEGF (25 and 250 pM) under the same conditions as above, there was no additional increase in the number of ephrinB2 positive cells beyond that achieved with 2.5 pM VEGF. What is a plausible explanation for this result? Only a limited percentage (~50%) of the undifferentiated ECs have functional receptor molecules in the PM capable of responding to expgenous VEGF....
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