Lecture 12 - Quantum mechanics

Lecture 12 - Quantum mechanics - max = 400 nm Colorimetric...

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Unformatted text preview: max = 400 nm Colorimetric Enzyme Assay colorless Beers Law: A = ( conc)(pathlength) A = log (I 0 /I) I I t V t S S t P max ) ]( [ ] [ ) ]( [ = = max ] [ ) ]( [ S t V t S + = Enzyme Inhibition Reversible vs Irreversible Inhibition: E + I EI E + I EI I forms covalent bond with E (e.g. nerve gas and cyanide etc.) I binds noncovalently (drugs, Hg2+, metabolites etc.) Negative feedback inhibition Drugs act as enzyme inhibitors: Tumor suppressors (Ras GTPase, tyrosine kinase etc.) Antivirals (HIV reverse transcriptase, HIV protease) Non-Competitive (decrease V max ) Uncompetitive (decrease K M and V max ) Types of Enzyme Inhibitors Competitive (increase K M ) + = ] [ ] [ 1 max I M K I V K slope ] [ ] ][ [ EI I E K I = [E] = [E] + [ES] + [EI] ] [ ] [ ] ][ [ ] [ 2 1 1 = = ES k ES k S E k dt ES d I K I E EI ] ][ [ ] [ = ) ] [ 1 ( ] [ ] [ ] [ I K I ES E E + = 1 1 2 1 1 ] [ 1 ] [ ] [ 1 ] [ ] [ ] [ + + + + = I I K I S k k k K I S E k ES ] [ ] [ 2 ES k dt P d v = = y = mx + b Competitive Inhibition ] [ ] [...
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This note was uploaded on 04/06/2011 for the course CHEM 107B taught by Professor Jamesames during the Winter '09 term at UC Davis.

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Lecture 12 - Quantum mechanics - max = 400 nm Colorimetric...

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