Lecture 26 - Spectroscopy

Lecture 26 - Spectroscopy - Final Exam Fri, Mar 18, 2011...

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Unformatted text preview: Final Exam Fri, Mar 18, 2011 10:30 12:30 pm 198 Young (last name begins with A- M) 176 Everson (last name begins with N- Z) Chapters 2.6 2.9, 9, 10.1 10.5, 11, 12.1 12.7, 13.1 13.4, 14.1, 14.3 ? Closed Book and Closed Notes No electronic devices except calculator At least one assigned homework problem will appear on the exam. Review session on Thu Mar 17 at 6:00 pm in 1100 Social Sciences. Practice Final Exam on website. Electronic Spectroscopy Electronic spectroscopy measures energy of electrons in bonds. UV or visible light energy excites electronic transitions. Born-Oppenhiemer Approximation: Electronic transition much faster than movement of the nuclei (decoupled) : Franck-Condon Principle: Vertical transition from v=0 initial state. Most probable transition maximizes overlap between final and initial vibrational wavefunctions. Franck-Condon Factor (selection rule): ) ,... ( ) ,... ( ) ,... , ,..., ( 1 1 1 1 m vib n el m n R R r r R R r r = 2 2 d S i vib f vib = replaces v = 1 v=0 v=6 v=4 v=0 Electronic Spectroscopy of Proteins UV absorption spectra of aromatic amino acids Trp Trp Tyr Tyr n n 280 280 280 + = pathlength conc protein A = 280 280 -> * ++++++ +++--- ++0--0-+0-+0 ++-++- +-+-+- * Protein concentration: The extinction coefficient of a protein at 280 nm can be calculated as = n tyr *2000 M-1 cm-1 + n trp * 5500 M-1 cm-1 . The protein calmodulin has three tyrosine and zero Trp. Calculate the protein concentration (in mg/ml) of a solution if A 280 is measured to be 0.1 using a 1 cm pathlength cuvette....
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Lecture 26 - Spectroscopy - Final Exam Fri, Mar 18, 2011...

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