L1 Practice problems

L1 Practice problems - MCB121W09 S Burgess Lecture 1...

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MCB121W09 S. Burgess Lecture 1 Practice problems 1. Shown below is the partial output of a 454 sequencing run. Write the base sequence of the first 15 nucleotides of the synthesized strand of DNA in the 5’-3’ direction. The flow order of nucleotides is TACG. 2. Name one way that LINE and SINE elements are similar and one way they are different 3. One gram of cultured human cells contains roughly 10 9 cells. The diploid human genome comprises 6.4 x 10 9 base pairs. If base pairs occur at an interval of 0.34 nm, how long is the total length of DNA in this sample in kilometers? 4. The number of genes encoded by the human genome is similar to the number of genes in the nematode Caenorhabditis elegans (~20,000). The size of the human genome in base pairs, however, is about 300 times greater. List three types of DNA sequence elements that may be overrepresented in the human genome to account for the differences in genome size between the two organisms.
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KEY 1. Shown below is the partial output of a 454 sequencing run. Write the base sequence of
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