L3-4 practice problems

L3-4 practice problems - MCB121 W09 Practice problems for...

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MCB121 W09 Practice problems for Lecture 3-4 1. The PCNA clamp has a donut-shaped structure with a center hole large enough to encircle a DNA double helix and a layer of water molecules. This protein can also interact with DNA polymerase. These two features combined serve to increase processivity of pol δ. How might these two features contribute to increasing the processivity of pol δ ? 2. DNA replication of chromosomal DNA is semi-conservative. What is the meaning of this term? 3. Briefly describe one method you could use to measure the rate of DNA synthesis in a cell. 4. List six enzymatic or non-enzymatic activities acting at or near the replication fork during replication. 5. Answer the following questions on telomeres: A. Telomerase is an RNP that adds many repeats of a specific DNA sequence to the 3’ ends of linear chromosomes. The RNA component of telomerase provides the template for this addition. If the sequence of the telomerase RNA is 5’CUAACCCUAA3’, what is the repeated sequence that is found on the lagging strand of the chromosome after telomere addition? Be sure to note the polarity. B. Telomeres share many features in common with a DNA a double-strand break, however they are not repaired as such. Describe one mechanisms that prevents telomeres from fusing to one another. You can use a diagram if it helps. 6. Draw the nucleic acids of a replication fork, identifying and labeling newly synthesized strands by name, labeling all relevant 5’ and 3’ ends, indicating the direction of fork movement with an arrow, and identifying the location of any RNA by use of a crooked line.
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7. You are investigating DNA synthesis in a mammalian tissue culture line and perform the following analysis. You synchronize the cells by arresting the cells in G1 of the cell cycle (prior to
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L3-4 practice problems - MCB121 W09 Practice problems for...

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