Lecture 4 MCB121

Lecture 4 MCB121 - I. Origins of replication A....

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
I. Origins of replication A. Identification of orgins-genetics 50-100,000 origins of rep in human genome, only a couple dozen have been identified because in humans there is no consensus sequence (sequence in common) ~150 Kb between origns, heterogeneous distribution -replication is by birectional which means that PIC 1 -in actively transcribed regions the origins will fire early which will be in our euchromatin, wheras silenced heterochromatin (those origins will fire late). Different part of genome aer replicating at differ times. -can be seen about how the neculeus are organized. Heterochromatin tends oto be on the outside while euchromatin are on the insde. PIC 2 -in yeast the consensus sequence for an origin of replication ther is only 11 bp (they form consensus sequencey and acts as origins of replication. -Yeast genetic tools allowed for the discovery of sequences that define a replicator. -Replicator- entire set of cis acting sequences (contiguous dna sequence) that is sufficient to direct initiation of dna replication -used an in vivo assay on yeast plasmid was able to replicate itself and confer phenotype u can select for -if you have a plasmid with no ARS sequence (autnoomusly replicationg sequence) -a plasmid dna that can be introduced into easy cell. If it had an ARS ti will be able to replicate
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 3

Lecture 4 MCB121 - I. Origins of replication A....

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online