Lecture 6 MCB121

Lecture 6 MCB121 - -non autonomous – transpoase encoded...

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Lec 6 Transposons and chromosome rearrangements I. Genomic instability of repetitive elements A. Translocations -double strand breaks intiates genomic instability -chromosome 1 is the largest and Chromosome 21 is the smallest -tranlsocations crossing over??? B. Indels -unequal corssing over between sister chromatids (G2) -let’s say you have chromosome (2 sister choromatids) and have repeated elements along sister chromatid (PIC 1) -insertions and deletions and crossing over events C. Mechanism of homologous recombination -double holiday junction look online! II. Transposons -50% genome transponsons -transposition- transfer of chromosome segment from one part of a chromosome to another -transposon is a mobile DNA element -a transposase is an enzyme encoded by transposon that catalyzes -autonomous- all genetic info present
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Unformatted text preview: -non autonomous – transpoase encoded by another element-SINE (no transpoase) – short interspersed neuclear element which requires LINE elements which stands for long-interspersed nuclear elements and they are long because they contain transpoase-SINE sequence found in humans A. Types B. mechanism of “cut and paste” C. Retroviral integration (look at how HIV works)-HIV is a retrovirus. Retroviral-like elements latent virus. -Envelopt+glycoproteins-capsid ssRNA genome+reverse transcriptase-RNA and capsid released. cDNA-RNASE H degrades the strand of an RNA duplex-integrase acts as a transpoase to insert the HIV genome into the chromosome-LTR- long terminal repeats-target site duplication III. Immunoglobulin Gene rearrangement...
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