Unformatted text preview: Denature, mix the products and anneale. One of the annealing products is shown below. This is the only one that would function as a template for PCR with only the outside primers. A B C Fill in with DNA polymerase PCR Set up two PCR reactions with the primers indicated below. They will produce the indicated products that will include the desired mutation. Add outside primers and PCR to amplify the mutant product A B C A B C A B C PCR C. S. Gasser, U. C. Davis, 2008...
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This note was uploaded on 04/06/2011 for the course MCB 121 taught by Professor Gasser during the Winter '09 term at UC Davis.
- Winter '09