Expression Overview

Expression Overview - MCB121 Overview of Gene Expression C...

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MCB121 Overview of Gene Expression C. Gasser 2/7/2008 Reading: Watson – p. 377-380, 383-384, 410-412, 415-417, 457-458 (you can also review these topics in your BIS101 text). Problems: MBOC - problems 6-12 – 6-16, 6-17, 6-20 I. Overview of gene expression II. Bacterial gene expression A. Transcription and transcription signals 1. RNA polymerase = core ( α 2 , ß, ß’) + σ . 2. Polymerase recognizes genes by presence of specific promoter sequences. 3. Operator regions regulate gene expression. 4. Transcription terminates at specific sites where stem-loop structures form in RNA. B. Translation in prokaryotes 1. Requires ribosome, tRNA, aminoacyl-tRNA synthase and other factors. 2. Specific ribosome binding site proximal to AUG start codon. 3. Co-transcriptional, polycistronic mRNA common. III. Eukaryotic gene expression A. Three polymerases 1. RNA polymerase I (pol I) – transcribes rRNA precursor which is processed to 5.8S, 18S and 28S rRNA (insensitive to α -amanitin).
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This note was uploaded on 04/06/2011 for the course MCB 121 taught by Professor Gasser during the Winter '09 term at UC Davis.

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Expression Overview - MCB121 Overview of Gene Expression C...

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