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Unformatted text preview: Leucine Zipper (bZip, bCC) - Dimerization, Jun, Fos, GCN4, C/EBP, CREB, (Myc, Max, OCT-2) Helix-Loop-Helix - Dimerization and DNA binding, MyoD, Myc, Max, Ig enhancer-binding, daughterless Other - MADS, Fungal Finger (Zn Cluster), unclassified C. Activation domains Acidic, amphipathic helix - GAL4, GCN4, (AP1, JUN) Gln-rich - SP1, Antp, Ubx, Jun, AP2, SRF, OCT-1, OCT-2 Pro-rich - CTF/NF-1, (OCT-1, AP2, SRF, Jun) All appear to act by recruiting components of basal apparatus or chromatin modifying enzymes. III. Identification and Isolation of Transcription Factors A. Purification by standard biochemical methods, assay by footprint or gel retardation B. Genetic identification - mutations affecting a whole process C. Several viral oncogenes and their cellular homologs have been found to be transcription factors....
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- Winter '09