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Transcription Regulation IIb

Transcription Regulation IIb - represses gene expression G...

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MCB121 Regulation of Transcription II 2/19/2008 Reading: Watson p. 605-610. Blackwood EM, Eisenman RN. (1991) Max: A helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with Myc. Science , 251:1211-7 available on web page (Understand Figs. 3, 5, 6, 7). Problems: Understand Fig. 3, 5, 6, and 7 in Blackwood paper; problem set on transcription regulation (same problem set as for previous lecture); MBOC prob. 7-35, 7-49, 7-52. I. Myc/Mad/Max interactions A. Myc had basic HLH and L-Zip regions, regulates cell division, target genes were unknown. B. Definition of binding site C. Identification of heterodimerization partner by direct screening D. Specificity of Max interactions with Myc, DNA binding of heterodimer E. Regulatory network c-,N-,L- Myc / Max / Mad F. Mad-Max dimer recruits co-repressors, SIN3A and HDAC2 (histone deacetylase) -
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Unformatted text preview: represses gene expression G. Myc-Max dimer recruits proteins including histone acetyl transferases (HAT) - promotes gene expression II. Nuclear hormone receptors A. Glucocorticoid receptor (GR) Identified glucocorticoid regulated enhancer element Identify glucocorticoid receptor - define GRE 2 C2C2 Zn fingers for binding and homodimerization Dimerization and nuclear localization induced by hormone B. Other nuclear hormone receptors [thyroid hormone receptor (TR), progesterone receptor (PR), estrogen receptor (ER), etc.] identified by sequence similarity or function. CKII = phosphorylation site (Casein kinase II can phosphorylate) BR = basic region HLH = helix loop helix LZ = leucine zipper AR = acidic region...
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