MCB121 MT2 Winter 2011

MCB121 MT2 Winter 2011 - Name Key Last, First MCB121, Page...

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Name Key MCB121, Page 1 of 9 Last, First Gasser, Burgess © 2011, Charles S. Gasser MCB121 Second Midterm, Feb 18, 2010 Instructions: There are nine pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. Amino acid Abbreviations Amino acid Abbreviations Alanine Ala A Leucine Leu L Arginine Arg R Lysine Lys K Asparagine Asn N Methionine Met M Aspartic acid Asp D Phenylalanine Phe F Cysteine Cys C Proline Pro P Glutamine Gln Q Serine Ser S Glutamic Acid Glu E Threonine Thr T Glycine Gly G Tryptophan Trp W Histidine His H Tyrosine Tyr Y Isoleucine Ile I Valine Val V
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Name Key MCB121, Page 2 of 9 Last, First Gasser, Burgess © 2011, Charles S. Gasser Question Value Score 1 20 2 16 3 24 4 20 5 15 6 12 7 19 8 18 9 6 Total 150 1. (20) Eukaryotes have three classes of genes, types I, II and III. These are transcribed by three similarly named polymerases. For each of the following, circle the number corresponding to the gene class for which the statement is true. If it is true for more than one class, then circle all of the gene classes for which it is true. If it is true for none of the gene classes, circle “N”. I III N TFIID is necessary for transcription of this gene class. I II N TFIIIA participates in transcription of this gene class. I II III Transcription of this gene class requires sigma factor. I II N Helicase activity is necessary for initiation of transcription of this gene class. II N For a genes of this class, transcription terminates at a specific conserved sequence.
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Name Key MCB121, Page 3 of 9 Last, First Gasser, Burgess © 2011, Charles S. Gasser 2. (16) Shown below is the sequence of a newly identified transcription factor, TF121. 1 MAAFNFTCL FRNDHQL WEDVKNL FNIYNHL NEVIACL SST 2 3 _ RR SHRKRK WARH CSK AR PR NSPQWERPP SPP EP EP RPP AR __ QKLNPP EPPP TF121 protein, and versions of the protein with specific deletions were synthesized in an in vitro translation system and were then assayed for DNA binding activity. The normal, and deleted forms of the protein were also produced from mammalian expression vectors through transient expression in mouse cells. The transient expression assays were used to evaluate the ability of the different proteins to activate transcription of a target gene. The resulting data are given in the table below. Construct Binds DNA? Activates Transcription? Intact TFMCB Yes Yes Region 1 deletion No No Region 2 deletion No No Region 3 deletion Yes No a. (12) Write the ONE letter corresponding to the most likely function of each region of the protein next to the number designating the domain.
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MCB121 MT2 Winter 2011 - Name Key Last, First MCB121, Page...

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