MCB121 PFinal Winter

MCB121 PFinal Winter - Name Last, First MCB121, Page 1 of...

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Name MCB121, Page 1 of 13 Last, First Gasser, Burgess MCB121 W11, Practice Final Exam Instructions: There are 11 pages in this exam including the cover sheet, please count them before you start to make sure all are present. Write your name on each page of the exam. Write your answers in the space provided below each question. If you need more space use the back of the page and indicate clearly that you have continued your answer on the back. Do not use additional paper. Amino acid Amino acid Abbreviations Alanine Ala A Leucine Leu L Arginine Arg R Lysine Lys K Asparagine Asn N Methionine Met M Aspartic acid Asp D Phenylalanine Phe F Cysteine Cys C Proline Pro P Glutamine Gln Q Serine Ser S Glutamic Acid Glu E Threonine Thr T Glycine Gly G Tryptophan Trp W Histidine His H Tyrosine Tyr Y Isoleucine Ile I Valine Val V Question Value Score 1 23 2 24 3 23 4 5 5 13 6 12 7 8 8 16 9 6 10 14 11 12 12 8 13 20 14 16 Total 200
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Name MCB121, Page 2 of 13 Last, First Gasser, Burgess 1. (23 points) You have identified a new member of the human nuclear hormone receptor family. You name it “Kryptonite Receptor” (KR) for its ability to bind the novel small molecule Kryptonite (K). In further work you find that KR appears to be able to regulate genes that include the sequence “ATAGNNCTAT” in their promoter regions. You name this sequence the “kryptonite regulatory element” or KRE. To study how KR regulates gene expression you perform gel shift assays to evaluate interactions between purified KR, a 32 P-labeled oligonucleotide including the KRE sequence, and two other purified proteins – BAF53 (a component of the SWI/SNF complex) and TAF II 250 (a histone acetyltransferase, HAT, that is also a component of TFIID). An autoradiogram of the results of this gel shift assay is shown below. All lanes include the labeled KRE oligonucleotide probe, and the presence or absence of other components is indicated by the “+” and “–“ signs, respectively. Use the above information to answer the following questions: a) (15) Circle T for “True”, F for “False” or N for “no evidence from this experiment” for each of the following. T F N KR can bind to co-repressors in the absence of K. T F N KR can only bind KRE when it is also bound to K. T F N TAF is lower in molecular weight than KR.
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Name MCB121, Page 3 of 13 Last, First Gasser, Burgess T F N BAF binds to KR in the absence of K. T F N KR appears to act in activation of target gene expression in the presence of K. b) (8) By which of the following mechanisms does the above experiment indicate that KR can affect transcription of target genes (circle all that apply). Remove methyl groups from cytosine residues in the promoter. Remove acetyl groups from the histones in the promoter region to close the chromatin.
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This note was uploaded on 04/06/2011 for the course MCB 121 taught by Professor Gasser during the Winter '09 term at UC Davis.

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MCB121 PFinal Winter - Name Last, First MCB121, Page 1 of...

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