MCB124_PROBLEM_SET2

MCB124_PROBLEM_SET2 - MCB 124 Enoch Baldwin Protein...

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MCB 124 Protein Quaternary Structure, Nucleic Acid Structure Enoch Baldwin PROBLEM SET II page 1 I. Quaternary Structure A. You are a Junior Researcher in Dr. Rico Mendation’s Lab. For your first project you are given a human protein from blood, GETNA, that has the following properties. 1) Using SDS/PAGE (denaturing electrophoresis), you see 2 bands of apparent molecular weight of ~30 kD and ~10 kD. You call these GETNA-1 and GETNA-2. 2) A partial peptide sequence allows you to identify the two cDNAs (cDNAs correspond to the spliced mRNAs, that is they contain no exons, only coding sequence), which each show a single reading frame. For GETNA-1, the reading frame is 885 bases, while for GETNA-2, the reading frame is 273 bases. The sequence of GETNA-1 implies that it is an ATPase (binds and hydrolyzes ATP). 3) Gel filtration chromatography, ultracentrifugation, and light scattering give similar results that depend on salt concentration and the presence/absense of ATP. a) In 150 mM NaCl/10 mM ATP, a single species of apparent molecular weight of 120 kD is observed. b) in 150 mM/2 mM ATP, two species are observed 120 kD and 40 kD. c) in 150 mM NaCl/noATP only a 40 kD species is observed. d) at 500 mM/20 mM ATP four species are observed, 120 kD, 40 kD, 30 kD and 10 kD. e) 500 mM NaCl/no ATP only two species are observed, 30 kD and 10 kD.
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This note was uploaded on 04/06/2011 for the course MCB 124 taught by Professor Baldwin during the Summer '09 term at UC Davis.

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MCB124_PROBLEM_SET2 - MCB 124 Enoch Baldwin Protein...

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