MCB124-PROBLEM_SET1

MCB124-PROBLEM_SET1 - MCB 124 Enoch Baldwin Protein Primary...

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MCB 124 Protein Primary, Secondary and Tertiary Structure Enoch Baldwin PROBLEM SET I page 1 I. AMINO ACIDS 1. Protein Sequencing Using only chemical cleavage, proteases, and carboxypeptidases (Table 1.5, Lecture1.pdf), Edman degradation, HPLC purification of peptides AND amino acid analysis, design a strategy to completely sequence the following peptide- HOWEVER because you are in poorly equipped lab, you can only get 3 residues from an Edman degradation. Ac-Met-Asp-Lys-Leu-Ala-Asn-Gly-Leu-Val-Glu-Arg-Phe-Leu-Lys-Ala-Arg-Arg 2. Amino acid properties Arrange the following amino acid dipeptides in order of Volume, Hydrophobcity Statistical tendency to be in a helix Charges at pH 2, pH 5.5 pH 10 (most positive to most negative) M-W P-G L-A H-I R-D A-V E-H W-K Q-E D-F A-G G-D H-K Y-T C-Y (Hint: see the tables in Lecture1.pdf) II. POLYPEPTIDES 1 . A) Sketch a tri-peptide Ile-Gly-Pro in the extended conformation . B) draw two boxes around the atoms contained within the two complete peptide planes C) indicate the bonds that correspond to the Gly phi and psi torsion angles which describe the relative positions of the two planes D) Draw a Ramachandran plot on back using the above as a guide. Use it to
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MCB124-PROBLEM_SET1 - MCB 124 Enoch Baldwin Protein Primary...

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