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Lecture 31. Fluorescence

Freeman why is the emitted light detected at a 90

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Unformatted text preview: Company, New York, 2001. Freeman Why is the emitted light detected at a 90º angle? angle? Fluorescence Fluorescence Fluorescence Fluorescence F = 2.303φ P0abc F: Fluorescence Intensity φ : Quantum Yield P0: Excitation Power c: Analyte Concentration a: Absorptivity (g-1 cm-1 L) Daniel C. Harris, Exploring Chemical Analysis, 2nd ed., W.H. Freeman and Company, New York, 2001. Freeman φ F = 1.0 φ F = 0.2 φ F = 0.8 not fluorescent Ingle and Crouch, Ingle Spectrochemical Analysis Spectrochemical Skoog, Hollar, Nieman, Principles of Instrumental Analysis, Principles Saunders College Publishing, Philadelphia, 1998. Saunders Absorbance and Fluorescence Characteristics Absorbance 10-1–10-13 Gary D. Christian, Analytical Chemistry, 6th ed., John Wiley & Sons, Inc., U.S.A., 2004. Why does fluorescence permit such low detection limits? detection...
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