Lecture_2 - How Cells are Studied 1. 2. 3. 4. 5. 6....

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How Cells are Studied 1. Isolation of cells 2. Sorting of cells 3. Primary cultures 4. Cell lines 5. Hybridomas 6. Cell fractionation
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Isolation of Cells Cell isolation involved disrupting the extracellular matrix Teasing out cells centrifugation differential plating
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Sorting of Cells FACS analysis (Fluorescence- activated cell sorter) highly sophisticated technique Uses antibodies coupled to fluorescent dye Lasers detect fluorescent signals
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Type of data from FACS analysis
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Primary Cultures Once you have isolated a cell = 1 o cell Cells can be maintained if given the right nutrients Cells can live outside the parent organism in tissue culture plates To a cell biologist these cells outside the body would be considered in vitro - this is different than to a biochemist say Whole fied of growing cells outside of organisms is called tissue culture
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This is where I plug my research!
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Myofibroblasts have roles in normal development and disease
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Myofibroblasts are involved in alveolar development
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Lung Development Requires Myofibroblasts Bostrom, H. et al (1996) Cell 85, 863-873.
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Myofibroblasts are involved in disease Idiopathic pulmonary fibrosis Lymphangioleiomyomytosis (LAM)
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What is IPF? Progressive inflammatory disease leading to scarring of
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Lecture_2 - How Cells are Studied 1. 2. 3. 4. 5. 6....

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