lecture_3 - Microscopy 1. Basic components 2. Resolution 3....

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Microscopy 1. Basic components 2. Resolution 3. Contrast a. Stains b. Phase-contrast microscopy 1. Fluorescence Microscopy 2. Electron Microscopy a. TEM b. SEM 1. Visualizing living cells and organisms
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Light Microscopy First type of Oculars Stage Diaphram Condensor Light source
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Resolution Optical quality of an image Amount of fine detail that can be discriminated Limit of resolution of a microscope is determined by diffraction Resolving power of a microscope is defined as : d= 0.61 λ /n sin θ Denominator is called the numerical aperture
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Resolution II Maximum NA for air = 1.0 Maximum NA for oil = 1.5 Common rule of thumb = useful magnification of a microscope is 500- 1000x the NA of the objective
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Contrast Real problem in optics Cells are translucent! 70% water Overcome with stains to make internal structures visible Increases contrast due to specificity for certain molecules Feuglen/hematoxylin - DNA and RNA Coomassie blue - protein
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This note was uploaded on 04/06/2011 for the course BIO 411 taught by Professor Nancya.rice during the Fall '09 term at Western Kentucky University.

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lecture_3 - Microscopy 1. Basic components 2. Resolution 3....

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