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Click to edit Master subtitle style Chapter 13 Chapter 13 CHAPTER 13. Biotechnology
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What Is
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Use or modification of organisms, cells, and biological molecules for practical purposes
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Goals of Biotechnology: To 1. Better understand inheritance & gene expression 2. 3. Generate social and economic benefits, including a. improved plants and animals for agriculture b. efficient production of valuable biomolecules
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How Does DNA Recombination
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Recombinant DNA Technology 1. Recombinant DNA a. Transfer of genetic material between organisms b. Same or different species 1. DNA recombination occurs naturally a. Sexual reproduction b. Bacterial transformation c. Viral transfer of DNA
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Plasmid: 1. Does not carry genes required for normal bacterial growth and survival 1. May carry genes that assist in survival and growth in certain environments; eg. Antibiotics
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1 µ m Recombination in Bacteria (Fig. 13-1) ( a ) ( b ) (c ) Bacteriu m Chromosome Plasmid Plasmid replicates in cytoplasm DNA fragment incorporated into chromosome Plasmid transferred to new host DNA fragments transferred to new host
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Viruses May Transfer Genes (Fig. 13-2)
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How Does DNA Recombination Occur in Genetic Engineering Laboratories?
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Restriction enzymes cut DNA at palindromes (A word, phrase, verse, or sentence that reads the same backward or forward)
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DNA Technology 1. Identify gene(s) of interest 2. Isolate the gene(s) of interest 3. Produce many copies of that gene 4. Use it
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1.
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Chapter_13_to_post - CHAPTER 13. Biotechnology Click to...

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