NOTES-Chapter 14

NOTES-Chapter 14 - Chapter 14: DNA synthesis, mutation,...

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Chapter 14: DNA synthesis, mutation, repair The most important point about DNA replication is that it must be accurate and to have some point mutations for genetic variation Models of DNA replication: o Conservative-both strands are conserved. One totally new DNA, one old DNA o Semi-conservative- one strand of new DNA and one strand of old DNA o Dispersive- have parts of both old and new DNA on both strands Meselson-stahl experiment o Grow E. Coli cells in medium with isotope 15 nitrogen as sole source of nitrogen b/c of nitrogenous bases. Collect sample and purify DNA o Transfer cells to medium containing isotope 14 nitrogen. After cells divide once, collect a sample and purify the DNA o After cells have divided a second time in isotope 14 nitrogen and collect sample and purify DNA o Showed that the model of replication is semi-conservative. DNA replication in vivo. NEED: o Template DNA-to copy from o Helicases- enzymes which break the Hydrogen bonds between the two strands and unwind the double helix of the DNA
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This note was uploaded on 04/07/2011 for the course BIOL 104 taught by Professor Bray-speth during the Fall '11 term at Saint Louis.

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NOTES-Chapter 14 - Chapter 14: DNA synthesis, mutation,...

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