BIOL3520Histology

BIOL3520Histology - Using commercially prepared slides...

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Histology,   Cytology, and     Histochemistry BIOL 3520 Nervous tissue
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Reminder First sectional exam next week! Covers the first THREE labs Micropipetting/error Microscopy Histology
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Tissue structure Histology: the study of tissue in thin section Microscopic anatomy Histochemistry: the use of chemical reactions to identify and manipulate tissue components Certain reactions interact characteristically with certain molecules
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Tissue slide preparation Preparation for microscopic investigation typically involves five main steps This is how the professional slides viewed last week were made Takes time! Fix Process Section Stain Mount Adipose tissue
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Today’s lab Prepare slides using a greatly abbreviated method that allows us to explore the main concepts within the lab period Obtain tissue sections (and a smear) Briefly fix and partially dehydrate the sections No clearing or embedding Fix after rather than before sectioning Apply a couple of common staining techniques View the slides (without proper mounting) under the microscope Examine the utility of enzyme histochemistry
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Unformatted text preview: Using commercially prepared slides Safety Some compounds are toxic, most are irritants Formalin is especially toxic Stains will dye almost everything they come in contact with Clothes, skin, etc. Wear proper protection, and be careful! Use clothespins to handle slides during staining process Feulgen stain Two major steps Treatment of tissue with warm HCl hydrolyses DNA, exposing reactive aldehyde groups Schiff reagent reveals this hydrolyzed DNA by binding the exposed aldehydes and producing a magenta color Feulgen stain Feulgen Staining of tumors in mice. Stained nuclei General tissue staining Stains may be applied alone or in combination to generally accentuate structure Haematoxylin: stains nuclear material blue Eosin: stains cytoplasmic material shades of red Methyl blue: stains collagen blue Enzyme histochemistry Enzymes react specifically with substrate By comparing staining patterns before and after enzymatic treatment, the target substrate can be identified/localized in the cell/tissue...
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This note was uploaded on 04/10/2011 for the course BIOL 3520 taught by Professor Staff during the Spring '11 term at North Texas.

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BIOL3520Histology - Using commercially prepared slides...

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