BIOL3520Histology - Histology Cytology,and Histochemistry...

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Histology,   Cytology, and     Histochemistry BIOL 3520 Nervous tissue
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Reminder First sectional exam next week! Covers the first THREE labs Micropipetting/error Microscopy Histology
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Tissue structure Histology: the study of tissue in thin section Microscopic anatomy Histochemistry: the use of chemical reactions to identify and manipulate tissue components Certain reactions interact characteristically with certain molecules
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Tissue slide preparation Preparation for microscopic investigation typically involves five main steps This is how the professional slides viewed last week were made Takes time! Fix Process Section Stain Mount Adipose tissue
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Today’s lab Prepare slides using a greatly abbreviated method that allows us to explore the main concepts within the lab period Obtain tissue sections (and a smear) Briefly fix and partially dehydrate the sections No clearing or embedding Fix after rather than before sectioning Apply a couple of common staining techniques View the slides (without proper mounting) under the microscope Examine the utility of enzyme histochemistry Using commercially prepared slides
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