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PRINT Conclusion Lab Unit 09 - Control by Heat and UV Light

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Conclusion Lab Unit 09 - Control by Heat and UV Light Levine 1 Alexis Levine Dr. Maxwell T/R 28, March 2010 In ninth unit, we examined two different ways to control microbial growth in a lab setting. The two ways we used to control microbial growth were exposing the microorganisms to heat and UV light for a certain amount of time. Once we had completed exposing each group to the proper amount of time, we incubated for 48 hours. The two organisms we used for the experiments were Escherichia coli and Bacillus subtilis . Our results for the first part of the lab, were based on putting our bacteria in a hot bath for a certain time and temperature. After the incubation, we examined the growth for each test tube, which was assigned a different temperature and time it would be in the bath. We can conclude from this that even though they were both in the bath for ten minutes, B. Subtilis takes a higher temperature to be killed. This is because it is more resistant to heat from producing more endospores. Another way we tried to kill bacteria was way UV light. In the control by heat experiment, there was growth in one tube and no growth in the other, growth in both tubes, or no growth at all in either tube. The organisms that were used were E. coli and B. subtilis. In my individual test tubes, there was growth in the test tube that contained B. subtilis and no growth in the test tube that contained E. coli . My temperature was 80 degrees C for 20 minutes. In greater part of the remainder of the test tubes with different temperatures and times,
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