Print Pre-Lab Lab Unit 10 - Antibiotics & Antimicrobials

Print Pre-Lab Lab Unit 10 - Antibiotics &...

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Levine 1 This week in lab we will study the effects antimicrobials and antibiotics have on bacterial cultures. Antimicrobial susceptibility testing is when a microorganism is inaccessible from a patient. In microbiology they will typically perform this form of testing. Frequently, there is uncertainty about what the results signify and how they can be used to direct the treatment of the patient (University of Pennsylvania Medical Center, 2010). The objective of antimicrobial susceptibility testing is to forecast the in vivo achievement or malfunction of antibiotic treatment. These tests are performed in vitro , and determine the growth response of an inaccessible organism to a specific drug(s). The Kirby-Bauer method is used to qualitatively determine the susceptibility of a given bacterial strain to a battery of antimicrobial drugs. This method is used to test whether a certain bacteria is susceptible to specific antibiotics (University of Pennsylvania Medical Center, 2010). This is determined by being grown on agar plates in the company of thin wafers that have applicable antibiotics. If the microorganisms are receptive to a specific antibiotic, there is a zone of inhibition. The zone of inhibition is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is (University of Pennsylvania Medical Center, 2010). It signifies the degree of susceptibility of the organism to the drug. If the bacteria are
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This note was uploaded on 04/10/2011 for the course BIOLOGY 2300 taught by Professor Staff during the Fall '10 term at Georgia State University, Atlanta.

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Print Pre-Lab Lab Unit 10 - Antibiotics &...

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