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Unformatted text preview: becomes independent of [S] when [S]>>Km. 5) An enzyme that converts a single substrate to a single product has kinetics conforming to the Michaelis-Menton equation with a K m of 1 M and an initial velocity of 0.1 M-1 min-1 at [S] equal 100 M. What is the initial velocity when [S] equals: (A) 1 mM, (B) 1 M, and (C) 2 M 6) Fumarase has a molecular weight of 194,000 and is composed of four identical subunits, each with fully functional active sites. If V max is 5 mM min-1 at an enzyme concentration of 1 x 10-8 M, calculate the k cat for fumarase. 7) In the double-reciprocal plot shown in the gure below, 1/v versus 1/[S] is plotted in the presence of four different xed concentrations of compound I, a reversible inhibitor. Based on the data plotted below, what type of inhibitor is compound I? Explain your answer. What parameter(s) of the Michaelis-Menton equation is given by the intercept on the 1/v (Y) axis? A B...
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- Spring '10