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Unformatted text preview: becomes independent of [S] when [S]>>Km. 5) An enzyme that converts a single substrate to a single product has kinetics conforming to the MichaelisMenton equation with a K m of 1 M and an initial velocity of 0.1 M1 min1 at [S] equal 100 M. What is the initial velocity when [S] equals: (A) 1 mM, (B) 1 M, and (C) 2 M 6) Fumarase has a molecular weight of 194,000 and is composed of four identical subunits, each with fully functional active sites. If V max is 5 mM min1 at an enzyme concentration of 1 x 108 M, calculate the k cat for fumarase. 7) In the doublereciprocal plot shown in the gure below, 1/v versus 1/[S] is plotted in the presence of four different xed concentrations of compound I, a reversible inhibitor. Based on the data plotted below, what type of inhibitor is compound I? Explain your answer. What parameter(s) of the MichaelisMenton equation is given by the intercept on the 1/v (Y) axis? A B...
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 Spring '10
 Mr.

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