Lecture20

Lecture20 - Chapter 8 E NZYMES : B ASIC C ONCEPTS AND K...

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Unformatted text preview: Chapter 8 E NZYMES : B ASIC C ONCEPTS AND K INETICS Lecture 20 Wednesday Oct 15, 2008 1 V o = V max [ S ] [ S ] + K m This equation is a rectangular hyperbola Wednesday Oct 15, 2008 V o = V max [ S ] [ S ] + K m This equation is a rectangular hyperbola Wednesday Oct 15, 2008 2 Determining initial velocities Wednesday Oct 15, 2008 3 K m = [S] at which V o = 2 V max Wednesday Oct 15, 2008 4 K m and V max are usually determined by fitting data to the Michaelis -Menten Eqn using non-linear regression analysis 1 V = K m V max [ S ] + 1 V max Lineweaver-Burke Eqn Wednesday Oct 15, 2008 5 k 1 k 1 k 2 K d = [ E ][ S ] [ ES ] = k " 1 k 1 dissociation constant for the ES complex K m = k 1 k-1 + k 2 if k-1 >> k 2 then: K m = k 1 k-1 = K d a measure of the afFnity of the enzyme for substrate Wednesday Oct 15, 2008 6 high K m low K m Enzyme has low affinity for substrate Enzyme has high affinity for substrate Wednesday Oct 15, 2008 7 k 1 k-1 k 2 k-2 k 3 Reactions where there are multiple steps after formation of ES complex. K m is a more complex term with multiple rate constants. Rxn Above : product release ( k 3 ) is rate limiting and most of the enzyme is in the EP form at saturating substrate concentration. general rate constant for rate limiting step V max = k cat [ E T ] V max = k 3 [ E T ] for the reaction above Wednesday Oct 15, 2008 8 Turnover number is the number of molecules of substrate converted to product per molecule of...
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Lecture20 - Chapter 8 E NZYMES : B ASIC C ONCEPTS AND K...

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