Unformatted text preview: complexes and how are they similar to fungal FAS? 4) How many times does the ACP domain interact with/bind the Ketosynthase domain in each extension cycle (ie. each time the chain gets extended by 2 carbons)? What is the oxidation state of the carbon β to the thioester in each case (ie. methylene, α/β unsaturated, β-hydroxy, β-keto, carboxylate, etc.)? 5) There are two steps in the catalytic mechanism of thioesterase domains. What are these two steps? Which one involves association with ACP? Which one is rate-limiting?...
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- Spring '07
- Enzyme, FAS, mammalian FAS differ, fungal FAS, ACP prosthetic group