lecture_3 - Chapter 2 Osmosis and Diffusion Diff Osmosis...

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Chapter 2 i d D i f f i Osmosis and Diffusion •O s m o s i s occurs by random movement/diffusion of molecules into all regions that are accessible to each molecule. This occurs constantly and, at some point, there is an equal concentration of permeant species n both sides of a barrier. on both sides of a barrier. • Dialysis is a useful technique for separating small molecules from large ones (see following figure). • For example, ions and other small molecules can be removed from a solution of protein via (repeated) dialysis 1
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Chapter 2 smosis and Diffusion Osmosis and Diffusion •O s m o s i s is the movement/diffusion of molecules through a barrier s m o s i s occurs so as to equalize the concentration of ermeable species on permeable species on both sides of barrier 2
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Chapter 2 Chemical properties of water • Ionization of water –K , pH • Acid-base chemistry – pK, Henderson-Hasselbalch equation • Buffers – titration curves 3
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Ionization of Water H 2 O H + + HO - • H+ (proton), HO- (hydroxide), H 3 O+ (hydronium ion) O - l l t h t K i d i i t i t t d [ A ] t t h     O H HO H 2 K Recall that K is an dissociation constant, and [A] represent the concentration of species A • Recall also that [H O] is 1M by definition, so the equation simplifies [ 2 ]y ,q p to: 4 - w HO H K
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Ionization of Water H 2 O H + + HO - • Pure water has equal concentrations of H+ and HO- • [H+]=[HO-]=(K ) 1/2 =10 -7 M for a neutral solution w • If [H+]> 10 -7 M, then the solution is acidic • If [H+]< 10 -7 M, then the solution is basic [] , • pH = -log[H+] 5
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Acid-Base Chemistry HA + H 2 O H 3 O + + A -
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This note was uploaded on 04/09/2011 for the course BCHS 3304 taught by Professor Johnson during the Spring '08 term at University of Houston.

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lecture_3 - Chapter 2 Osmosis and Diffusion Diff Osmosis...

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