Lab 9 - Lab 9 TCA(Trichloroacetic Acid precipitation of...

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Lab 9: TCA (Trichloroacetic Acid) precipitation of proteins The design of an appropriate procedure for isolation of a given protein should be tailored in accordance with the objective(s) of the research project, which may require relatively pure product in modest amounts for analytical purposes (e.g. enzyme kinetics) or a highly purified, homogeneous preparation for physicochemical or structural studies. Isolation and purification of a single protein from cells containing a mixture of thousands of unrelated proteins is achievable because of the remarkable variation in the physical and chemical attributes of proteins. Characteristics unique to each protein such as, amino acid composition, sequence, subunit structure, size, shape, net charge, isoelectric point, solubility, heat-stability, hydrophobicity, ligand/metal binding properties and post-translational modifications can be exploited in formulation of a strategy for purification. Based on these properties a combination of various methods, listed below, can be used for separation of cellular proteins.
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