Lecture 10 - Gene Structure and Transcription Terminology...

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Unformatted text preview: Gene Structure and Transcription Terminology • Genome – entire genetic material of an individual • Transcriptome – set of transcribed sequences • Proteome – set of proteins encoded by the genome • Only one strand of DNA serves as a template for transcription. • Different genes are transcribed from different strands From Gene to Protein Prokaryotic Gene Structure Promoter CDS Terminator transcription Genomic DNA mRNA protein UTR UTR translation Eukaryotic Gene Structure 5’ - Promoter Exon1 Intron1 Exon2 Terminator – 3’ UTR splice splice UTR transcription translation Poly A protein • Gene: a segment of DNA containing the information for a single polypeptide chain or functional RNA (i.e. rRNA, tRNA, etc.) Basic Gene Structure transcription 5’ ACAU…AUG…UGA…AUGA 3’ RNA Transcribed region Regulatory elements 5’ ACAT…ATG…TGA…ATGC 3’ 3’ TGTA…TAC…ACT…TACT 5’ Promoter Terminator DNA Gene +1 Downstream (+n) Upstream (-n) RNAP • The site at which RNA polymerase begins transcription is numbered +1. - Downstream: direction in which a template DNA strand is transcribed - Upstream: denotes the opposite direction - Nucleotide positions in the DNA sequence downstream are indicated by (+) and those upstream (-). Which DNA strand is the template strand? Gene Structure: Polycistronic vs monocistronic • Cistron : an old name for a gene • Polycistronic : 1 promoter directs synthesis of 1 mRNA that can be translated to more than one polypeptide – Prokaryotic genes • Monocistronic : 1 promoter directs synthesis of 1 mRNA that usually translates to only 1 protein – Eukaryotic genes Transcription...
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This note was uploaded on 04/09/2011 for the course CHEM 4461 taught by Professor Max during the Spring '08 term at Lamar University.

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Lecture 10 - Gene Structure and Transcription Terminology...

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