ComparisonPCRandreplicationkey - PlaceforDNA polymeraseto...

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Comparison between PCR and Human DNA replication   Feature in humans Feature in PCR Place for DNA  polymerase to  attach to DNA  strand RNA primers DNA primers Separates the  two strands of  DNA Helicase Heat Name of enzyme  that elongates  new strand of  DNA DNA  polymerase Taq or other  thermophillic  DNA  polymerase What the primers  are made out of  (DNA or RNA?) RNA DNA Items that are  common in both  situations that  has not already  been mentioned. Nucleotides  are needed for  elongation,  DNA serves  as template  for new strand Nucleotides are  needed for  elongation,  DNA serves as  template for  new strand What goes in the microfuge tube?
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Template strand- from the cheek cell  DNA- your DNA Primers- Primer mix Master mix:  Taq polymerase Nucleotides Buffer There are three major temperature changes that go on in one cycle of a PCR  reaction.  State the relative temperature and what happens at that step.
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This note was uploaded on 04/09/2011 for the course CHEM 4461 taught by Professor Max during the Spring '08 term at Lamar University.

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ComparisonPCRandreplicationkey - PlaceforDNA polymeraseto...

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